Deleted
Sample Discontinued
Experience: None
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Composite

Part:BBa_K875000:Design

Designed by: Federico Colombo, Elisa Clagnan   Group: iGEM12_Trieste   (2012-09-23)


B-Glucosidase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We made a PCR on Bba_K392008 by using the following primers: β-Glucosidase_Fw GCATGAATTCGCGGCCGCTTCTAGATGACCACCACGCGCCCCTC β-Glucosidase_Rev GCATCTGCAGCGGCCGCTACTAGTATCAGGGCTGGTAGGTCGCGG



Source

We made this BioBrick using others parts already present in the Registry of standard parts.

References